Technology

State Of The Art Infrastructure at Access Sites

A total infrastructure of over 90 state-of-the-art mass spectrometers are available in eleven transnational access sites.

Over 30 Orbitrap’s, more than 10 Q – TOF’s, and  5 Triple Quad’s. The platform also houses a series of MALDI-TOF/TOF’s and FT-ICR high resolution instruments. Different access sites will host projects with different requirements (e.g. PTM analysis, membrane protein analysis, Bio-imaging, native structure analysis, native MS).

UTRECHT UNIVERSITY

UNIVERSITY OF COPENHAGEN

TECHNOLOGY

Shotgun proteomics

  • 2x Q-Exactive HF-X
  • 1x Q-Exactive HF
  • 1x Fusion Lumos
  • 1x Fusion
  • 1x Orbitrap-XL
  • 3x triple-quad

Native / top-down proteomics

  • 2x UHMR
  • 8x QTOF type instruments

Sample preparation

  • Agilent BRAVO
  • SEC / SCX / high-pH / Mixed-phase

Software

  • XlinkX in Proteome Discoverer 2.2/2.3

TECHNOLOGY

Shotgun proteomics

  • 10x Q-Exactive HF-X
  • 1x Orbitrap Fusion Lumos

Online LC-MS/MS

  • 12x Easy nLC1200
  • 2x Easy nLC1000
  • 3x EvoSep OneLC

 

The instruments include 10 of the latest generation of Q Exactive HF-X mass spectrometers exhibiting improved speed and sensitivity, and one Orbitrap Fusion Lumos mass spectrometer providing complementary fragmentation techniques and scanning techniques, such as ETD and MS3 acquisition.

ETH/ FUNCTIONAL GENOMICS CENTER                                                       ZURICH

TECHNICAL UNIVERSITY OF MUNICH

TECHNOLOGY

Mass Spec Instruments 

  • 8x mass spectrometers
  • 1x Orbitrap

 

This facility houses nine mass spectrometers, which are equipped for nanoLC-MS/MS proteomics-type experiments.

The instruments include state-of-the-art technologies like high-resolution Orbitrap mass spectrometry, CID, HCD, electron transfer dissociation (ETD), and combinations thereof (EThcD), and cross-linking MS.

Collectively, the analytical technologies provide a full-spectrum infrastructure for high throughput and in-depth proteome analysis. Based on the broad fundament of methodological expertise and know-how, also targeted MS-based approaches, including selected reaction monitoring (SRM) and parallel reaction monitoring (PRM), as well as data independent acquisition (DIA)

TECHNOLOGY

Mass Spectrometers

  • 4x Orbitrap Lumos,
  • 3x Orbitrap HF-X

Expertise

  • DDA (SILAC, TMT, label-free)
  • DIA (PRM, SWATH)
  • Bottom up proteomics
  • Full proteomes
  • Phosphoproteomes, PTMs
  • Affinity proteomes
  • Tissues, body fluids

 

The infrastructure boasts the latest hardware for proteomics including three Orbitrap Fusion Lumos instruments (equipped with electron-transfer dissociation, ETD or ultra-violet photo-dissociation, UVPD) and three Orbitrap Q-Exactive HF-X instruments. The Orbitrap instruments are furnished with ultra-high performance nano-liquid chromatography systems. This outstanding equipment base enables proteomic projects at any scale and ranging from high-throughput discovery (data-dependent acquisition, DDA), targeted (parallel reaction monitoring, PRM) and data-independent (DIA) experiments.

 

VLAAMS INSTITUUT VOOR BIOTECHNOLOGIE (VIB), GHENT, BELGIUM

PASTEUR INSTITUTE, FRANCE

TECHNOLOGY

Mass Spec Instruments 

  • 6 x mass specs including several Orbitraps
  • 1x Triple Quad
  • 1x MALDI MS/MS

This facility houses 8 mass spectrometers, all coupled to sensitive nano-LC chromatography systems.

In combination with PSD, CID, HCD and ETD peptide fragmentation possibilities, these instruments enable high-throughput analysis of complex proteome samples as well as intact protein measurements. In addition, workflows for targeted MS-based approaches, including selected/parallel reaction monitoring (SRM/PRM) and data independent acquisition (DIA) are in place.

TECHNOLOGY

Mass Spec Instruments 

  • 1x Orbitrap LTQ-Velos (ETD)
  • 1x Synapt G2SI & HDX Manager
  • 1x Orbitrap Fusion Lumos (ETD, UVPD)
  • 1x Q-Exactive HF
  • 2x Q-Exactive plus

The instruments include state-of-the-art technologies like CID, HCD, Surface Induced Dissociation (SID), Electron Transfer Dissociation (ETD), and combinations thereof (EThcD), Ion Mobility Separation (IMS), cross-linking MS and H/D exchange MS (with an automated HDX platform). Ultra-violet photo-dissociation (UVPD) will be available soon.

CENTRE FOR GENOMIC REGULATION (CRG)/

                              POMPEU FABRA UNIVERSITY (UPF), SPAIN

SPANISH NATIONAL CANCER                                                                         RESEARCH CENTRE

TECHNOLOGY

Mass Spec Instruments 

  • 1 x Orbitrap Fusion Lumos
  • 1x LTQ Orbitrap XL
  • 1x LTQ ORBITRAP Velos
  • 1x QTrap 5500
  • 1x QTOF 5600

All mass spectrometers are equipped with nanoLC chromatographic systems for quantitative proteomics experiments, and they cover a wide range of mass analysers (orbitrap, TOF, quadrupoles, ion traps), fragmentation types (CID, HCD, ETD, and their combinations), and acquisition workflows (e.g. DDA, SRM, PRM, and DIA).

TECHNOLOGY

Mass Spec Instruments 

  • 2 x Qex HF-X
  • 1x Qex Plus
  • 1x Bruker Impact QqTOF
  • 1x LTQ Orbitrap Velos
  • 1x QTrap 5500

 

NanoLC chromatographic systems are coupled to these instruments  for quantitative proteomics experiments, and they cover a wide range of mass analysers (orbitrap, TOF, quadrupoles, ion traps), fragmentation types (CID, HCD, ETD, and their combinations), and acquisition workflows (e.g. DDA, SRM, PRM, and DIA).

 

     

     INSTITUTE OF MICROBIOLOGY, CZECH ACADEMY OF SCIENCES, VESTEC/MASARYK UNIVERSITY, BRNO, CZECH REPUBLIC

                                     

CEITEC MASARYK UNIVERSITY, BRNO,                                                 CZECH REPUBLIC

TECHNOLOGY

Mass Spec Instruments 

  • 2x FT-ICR

These facilities jointly house two FT-ICR mass spectrometers (Bruker solariX Ultra-high Resolution Hybrid Qh-Fourier Transform Mass Spectrometers). Both 12 Tesla and 15 Tesla instruments are equipped with a MALDI/ESI dual source, ETD fragmentation module and ParaCell.

The 15 T instrument is equipped with an infrared laser for infrared multiphoton dissociation. Nano RSLC 3000 (ThermoFisher Scientific) and Agilent 1200 (Agilent Technologies) HPLC systems and PAL autosampler/robot (CTC analytics), MALDI-TOF/TOF MS (Ultraflex III, Bruker Daltonics) and IMMS Q-TOF (Synapt G2 HD, Waters)

TECHNOLOGY

Mass Spec Instruments

  • 1x MALDI-TOF/TOF MS
  • 1x Orbitrap Fusion Lumos
  • 1x Orbitrap Elite.

LC MS/MS experiments are provided by the coupling of  nano LC chromatographic systems to an Orbitrap Fusion Lumos and an Orbitrap Elite.

EUROPEAN INSTITUTE OF ONCOLOGY, ITALY

TECHNOLOGY

Mass Spec Instruments 

  • 1 x Q Exactive HF Orbitrap
  • 1x Q Exactive Plus

Sample prep

1x OFFGEL fractionator, 3100 Agilent

This facility operates one off-line HPLC system and two dedicated ultra-high-pressure liquid chromatography (UHPLC) systems coupled with a high field hybrid quadruple-orbitrap mass spectrometer (QExactive-HF) and has additional access to an identical instrument setup is also available at the centralized Consortium (COGENTECH) of the Campus, when additional measurement is needed.

This MS platform is integrated to an ample set of consolidated biochemical and analytical protocols for histone, protein, peptide, modified peptide and sub-proteomes purification prior to MS-analysis. Standard Data-dependent acquisition (DDA) methods are normally used, but hybrid DDA-single reaction monitoring (SIM) can also be employed to specifically target modified peptides in complex matrices.

Genomic Unit

HiSeq 2000, Miseq, NovaSeq 6000, Nanostring nCounter, Biomek FX, LabChip GX; Bioanalyzer 2100, TapeStation, 10X Genomics Chromium, Covaris S220 series, to perform WGS, WES, RNAseq, ChIP-Seq, RepliSeq, Methylation, single-cells RNA analyses.

Crystallography Unit

The Crystallography Unit offers Static Light Scattering, Isothermal Titration Calorimetry, Thermofluor, Mosquito nanodispenser, Crystal Imager for protein expression in insect cells (baculovirus), Biochemical characterization of macromolecular complexes and their affinities, Crystallogenesis, X-ray diffraction data collection and structure determination.

Imaging Unit

The Imaging Unit is equipped with Fluorescence and Confocal Microscopes (including FRAP, FRET, Photoactivation, Tissue Mosaic Reconstruction), Robotized microscopy for high content and resolution image cytometry, to offer services like Basic Image acquisition and Widefield Timelapse, Tissue and Organoids Fluorescence Imaging, Live Cell Imaging (Confocal Timelapse, FRAP, Photoactivation, FRET), Deconvolution (Widefield), in addition to Training and assistance for Users

                 ROYAL INSTITUTE OF TECHNOLOGY, STOCKHOLM       

CELL PROFILING TECHNOLOGY

  • 25,000 validated antibodies
  • Several flouresence microscopes
  • Tools for highly multiplex imaging

The expertise of the Cell Profiling facility is in antibody based imaging to study spatial proteomics in situ. The Cell Profiling facility offers a broad range of infrastructure services within the following pillars; proteomics, bioimaging and molecular structure, single cell biology, genomics, metabolomics, chemical biology and genome engineering, bioinformatics, drug discovery and diagnostics.